Children suffering from infectious diseases, both bacterial and viral, are often treated with empirical antibiotics. Keeping in mind both the menace of microorganisms and antibiotic toxicity, it is imperative to develop point-of-care testing (POCT) to discriminate bacterial from viral infections, and to define indications for antibiotic treatment. This article reviews potential protein biomarkers and host-derived gene expression signatures for differentiating between bacterial and viral infections in children, and focuses on emerging multiplex POCT devices for the simultaneous detection of sets of protein biomarkers or streamlined gene expression signatures that may provide rapid and cost-effective pathogen-discriminating tools.Microglia have long been considered a homogenous cell population that uniformly responds to extrinsic factors. Here, we describe how the recent development of single-cell technologies has revealed the heterogeneity of both human and mouse microglia and identified distinct microglial states linked to specific developmental, aging, and disease stages. We discuss progress and future developments in data analysis, essential tools for the comprehension of big data derived from single-cell omics, and the necessity of integrating such data with functional studies to correlate genetic cues with the relevant biological functions of microglia. Defining the functional correlates of distinct microglia states is fundamental to dissecting the 'microglial etiology' of aging and complex neurological diseases and identifying novel therapeutic and diagnostic targets.
Patients awaiting heart transplantation (HTx) often need bridging therapies to reduce worsening and progression of underlying disease. Limited data are available regarding the use of the MitraClip procedure in secondary mitral regurgitation for this clinical condition.
We evaluated an international, multicenter (17 centers) registry including 119 patients (median age 58 years) with moderate-to-severe or severe secondary mitral regurgitation and advanced heart failure (HF) (median left ventricular ejection fraction 26%) treated with MitraClip as a bridge strategy according to 1 of the following criteria (1) patients active on HTx list (in list group) (n = 31); (2) patients suitable for HTx but awaiting clinical decision (bridge to decision group) (n = 54); or (3) patients not yet suitable for HTx because of potentially reversible relative contraindications (bridge to candidacy group) (n = 34).
Procedural success was achieved in 87.5% of cases, and 30-day survival was 100%. At 1 year, Kaplan-Meier estimat-generating to guide further study for percutaneous intervention in high-risk patients with advanced HF.
Theatre actors use voice in virtually any physical position, moving or still, and perform in a wide range of venues. The present study investigated acoustic qualities required to perform classical material without electronic amplification in outdoor spaces.
Eight professional actors, four female, four male, from NY Classical Theatre performed one-minute monologues, first stationary, then moving, for audio recording in Central Park. Four subjects recorded two monologues each, from productions in which they played both male and female characters. Data were analyzed for fundamental frequency (F0), sound pressure level (SPL), and long-term average spectrum (LTAS).
Overall, F0 ranged between 75.38 and 530.33 Hz. Average F0 was 326 Hz stationary and 335.78 Hz moving for females, 248.54 Hz stationary, 252.82 Hz moving for males. SPL ranged from 28.54 to 110.51 dB for females, and 56.69 to 124.44 dB for males. Average SPL was 82 dB for females, 96.98 dB for males. On LTAS, females had a peak between 3 and 4 kHz ranging from 1.5 to 4.5 dB and another between 4 and 5 kHz ranging from 2 to 4.5 dB, while males had a peak between 3 and 4 kHz ranging from 1 to 8.5 dB.
Actors appear to use a similar F0 range across gender and performing conditions. Average F0 increased from stationary to moving. BGB-3245 Males had greater SPL values than females, and the amplitude of peaks in the region of the Actor's Formant of LTAS curves was higher in male than female voices.
Actors appear to use a similar F0 range across gender and performing conditions. Average F0 increased from stationary to moving. Males had greater SPL values than females, and the amplitude of peaks in the region of the Actor's Formant of LTAS curves was higher in male than female voices.
Abnormal esophageal motility is prevalent in gastroesophageal reflux disease patients; however, its relationship with laryngopharyngeal reflux (LPR) symptom severity remains unclear. Altered esophageal transit may contribute to LPR symptoms. We aimed to examine the relationship between reflux symptom index (RSI), a validated questionnaire for LPR symptoms, and abnormal esophageal motility on high-resolution manometry (HRM).
A total of 133 consecutive patients (55.9 ± 14.6 years, 69.9% female) with suspected LPR referred for HRM and multichannel intraluminal impedance-pH study (MII-pH) at a tertiary center from March 2015 to October 2017 were included. RSI questionnaire was prospectively collected prior to motility testing. Authors analyzing HRM and MII-pH were blinded to RSI findings. Statistical analyses were performed using Student's t test or Pearson's correlation (univariate) and general linear regression (multivariable).
Mean RSI was higher among patients with ineffective esophageal motility than tfter controlling for reflux on MII-pH. Esophageal dysmotility may play a role in suspected LPR symptoms independent of reflux. HRM should be routinely considered in evaluating these patients.Ascariasis is a globally spread intestinal nematode infection of humans and a considerable concern in pig husbandry. Ascaris accomplishes a complex body migration from the intestine via the liver and lung before returning to the intestine. Tissue migration and the habitat shared with a complex microbial community pose the question of how the nematode interacts with microbes and host cells from various tissues. This review addresses the current knowledge of the trilateral relationship between Ascaris, its microbial environment, and host cells, and discusses novel approaches targeting these interactions to combat this widespread infection of livestock and man.BGB-3245
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